Review



plenticrispr v2 vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Addgene inc plenticrispr v2 vector
    Plenticrispr V2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenticrispr+v2+vector/lentiCRISPR+v2+(Plasmid+%2352961)/pm41747732-322-31-34
    Average 96 stars, based on 6181 article reviews
    plenticrispr v2 vector - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: CYLD suppresses infectious Bursal disease virus replication by deubiquitinating VP1.
    Article Snippet: Cylindromatosis (CYLD), a deubiquitinase (DUB), removes K63-linked ubiquitin chains from idiosyncratic zymolytes, negatively regulating multifarious signaling channels, including NF-κ B, which is implicated in various cancers.. Recently, the role of CYLD in antiviral immunity has gained attention, but its involvement in infectious Bursal diseases virus (IBDV) infection remains unclear.. By coimmunoprecipitation combined with mass spectrometry analysis, we identified CYLD as a DUB bound to IBDV VP1.

    Article Title: Tetrandrine regulates NAADP-mediated calcium signaling through a LIMP-2-dependent and sphingosine-mediated mechanism.
    Article Snippet: .. Establishment of LIMP-2 knockout cell lines Two guide RNAs (gRNAs) (5′-CACCGATGCTGCTTCTACACGGCG-3′ and 5’-CACCGCTTCCTCAGGGAGATCATCG-3’) targeting exon 1 and 4 of LIMP-2 gene, respectively, were cloned into pLentiCRISPR-V2 vector (Addgene #52961). ..

    Article Title: Tetrandrine regulates NAADP-mediated calcium signaling through a LIMP-2-dependent and sphingosine-mediated mechanism
    Article Snippet: .. Two guide RNAs (gRNAs) (5′-CACCGATGCTGCTTCTACACGGCG-3′ and 5’-CACCGCTTCCTCAGGGAGATCATCG-3’) targeting exon 1 and 4 of LIMP-2 gene, respectively, were cloned into pLentiCRISPR-V2 vector (Addgene #52961). ..

    Plasmid Preparation:

    Article Title: CYLD suppresses infectious Bursal disease virus replication by deubiquitinating VP1.
    Article Snippet: Cylindromatosis (CYLD), a deubiquitinase (DUB), removes K63-linked ubiquitin chains from idiosyncratic zymolytes, negatively regulating multifarious signaling channels, including NF-κ B, which is implicated in various cancers.. Recently, the role of CYLD in antiviral immunity has gained attention, but its involvement in infectious Bursal diseases virus (IBDV) infection remains unclear.. By coimmunoprecipitation combined with mass spectrometry analysis, we identified CYLD as a DUB bound to IBDV VP1.

    Article Title: Neutrophil progenitor cell therapy rescues host defense against Staphylococcus aureus in murine chronic granulomatous disease.
    Article Snippet: .. 2 To generate the Ly6G-deficient NP cell line, we used the pLentiCRISPR v2 vector, a gift 3 from Feng Zhang (Addgene plasmid #52961) with the following 20-nucleotide single-4 guide RNA targeting sequence inserted: CACACAGTAGGACCACAAGA (Ly6g). ..

    Article Title: YIPF5 is an essential host factor for porcine epidemic diarrhea virus double-membrane vesicle formation
    Article Snippet: .. These sgRNAs were subsequently amplified and inserted into the plentiCRISPR v2 vector (Addgene plasmid #52961) at the BsmBI site using the Gibson Assembly method (New England Biolabs). .. Subsequently, the ligation products were electroporated into DH10B competent cells, and the bacteria were harvested for maxipreparation with the Endo-free Plasmid Maxi kit (Qiagen).

    Article Title: Neutrophil progenitor cell therapy rescues host defense against Staphylococcus aureus in murine chronic granulomatous disease
    Article Snippet: .. To generate the Ly6G-deficient NP cell line, we used the pLentiCRISPR v2 vector, a gift from Feng Zhang (Addgene plasmid #52961) with the following 20-nucleotide single guide RNA targeting sequence inserted: CACACAGTAGGACCACAAGA ( Ly6g ). ..

    Article Title: Syncytial death mediated by oncolytic rVSV-NDV dynamically activates immunogenic apoptosis and necroptosis in human lung cancer cells
    Article Snippet: Cells treated with the decoupling agent FCCP (2–50 μM, Cayman Chemicals) for 10 min were used as positive controls. .. The plentiCRISPR v2 vector (# 52961, AddGene), was kindly provided by Prof. Andreas Pichlmair (Institute of Virology, TUM). .. Caspase activity in infected cells was measured at defined timepoints using Caspase-Glo 3/7 Assay or Caspase-Glo 8 Assay (Promega) following the manufacturer’s instructions.

    Sequencing:

    Article Title: Neutrophil progenitor cell therapy rescues host defense against Staphylococcus aureus in murine chronic granulomatous disease.
    Article Snippet: .. 2 To generate the Ly6G-deficient NP cell line, we used the pLentiCRISPR v2 vector, a gift 3 from Feng Zhang (Addgene plasmid #52961) with the following 20-nucleotide single-4 guide RNA targeting sequence inserted: CACACAGTAGGACCACAAGA (Ly6g). ..

    Article Title: Neutrophil progenitor cell therapy rescues host defense against Staphylococcus aureus in murine chronic granulomatous disease
    Article Snippet: .. To generate the Ly6G-deficient NP cell line, we used the pLentiCRISPR v2 vector, a gift from Feng Zhang (Addgene plasmid #52961) with the following 20-nucleotide single guide RNA targeting sequence inserted: CACACAGTAGGACCACAAGA ( Ly6g ). ..

    Knock-Out:

    Article Title: Tetrandrine regulates NAADP-mediated calcium signaling through a LIMP-2-dependent and sphingosine-mediated mechanism.
    Article Snippet: .. Establishment of LIMP-2 knockout cell lines Two guide RNAs (gRNAs) (5′-CACCGATGCTGCTTCTACACGGCG-3′ and 5’-CACCGCTTCCTCAGGGAGATCATCG-3’) targeting exon 1 and 4 of LIMP-2 gene, respectively, were cloned into pLentiCRISPR-V2 vector (Addgene #52961). ..

    Amplification:

    Article Title: YIPF5 is an essential host factor for porcine epidemic diarrhea virus double-membrane vesicle formation
    Article Snippet: .. These sgRNAs were subsequently amplified and inserted into the plentiCRISPR v2 vector (Addgene plasmid #52961) at the BsmBI site using the Gibson Assembly method (New England Biolabs). .. Subsequently, the ligation products were electroporated into DH10B competent cells, and the bacteria were harvested for maxipreparation with the Endo-free Plasmid Maxi kit (Qiagen).

    other:

    Article Title: Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction.
    Article Snippet: 5,000 HEK293T cells per well were seeded on U-bottom Nunclon Sphera 96-Well plates (ThermoFisher, 174925) in 200 μL of glucose-free and glutamine-free DMEM supplemented with 25 mM glucose, 10% dialyzed FBS, and 100 U/mL penicillin/streptomycin.



    Similar Products

    96
    Addgene inc plenticrispr v2 vector
    Plenticrispr V2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenticrispr+v2+vector/lentiCRISPR+v2+(Plasmid+%2352961)/pm41747732-322-31-34
    Average 96 stars, based on 1 article reviews
    plenticrispr v2 vector - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc vector plenticrispr v2 0
    Vector Plenticrispr V2 0, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenticrispr+v2+vector/lentiCRISPR+v2+(Plasmid+%2352961)/pmc12488480-285-39-42
    Average 96 stars, based on 1 article reviews
    vector plenticrispr v2 0 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    94
    Addgene inc plenticrispr cas9 v2 vector
    Plenticrispr Cas9 V2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenticrispr+v2+vector/LentiCRISPR+v2+mBAX+(Plasmid+%23129577)/pmc06099880__pnas__1806239115__sapp-17-19-22
    Average 94 stars, based on 1 article reviews
    plenticrispr cas9 v2 vector - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results